Our lab is focusing on understanding the complexity of brain development and molecular mechanisms that awry in neurological and neurodevelopmental disorders with particular emphasis on myelination impairment, developmental epileptic encephalopathies, and ataxia. Recessive mutations in a single gene (example PRUNE1, EPT1 and DHX37) can impact the whole brain development and disease progression in children. How these genes are implicated in brain development and disease are largely unknown. We use primary cells (NSCs, Neurons and Oligodendrocytes), conditional mouse models and human brain organoids (derived from CRISPR edited ESCs or patient derived iPSCs) as model systems to dissect the cellular (cell autonomous and non-cell autonomous) and molecular events that intricate in brain physiology, and disease progression. We use cell biology, biochemistry, molecular biology and cutting-edge techniques (Proximity ligation, Mass Spectrometry, RNA-Seq, Chip-Seq and Proteomics) to delineate the disease mechanisms and find possible therapeutic interventions by regenerative medicine approaches