InStem Mass spec facility for Qualitiative and Quantitative charactetization of Biomolecules 

Zeno TOF 7600 - Zeno TOF 7600 is coupled high flow UPLC for analysis of metabolite, Protein, Peptide and Lipids. The 7600 Zeno TOF having the power of Zeno trap in combination with the binary fragmention mechnism i.e. Collision induced dissociaton and  EAD ( Electron activated dissociation) is very useful for the characterization of biomolecules. The high resolution of the analyzer is well suited for the characterization of biosimilars, Biobetter and intact proteins.Due to zeno trapping of the ion the sensitivity of this platfrom is enhanced multiple fold and dynamic range for the quantitation has also got improved significantly.

Following Mass Spectrometry Platfroms are present in the BLiSc cluster institutes.

Thermo Q-Exactive

5600 Triple TOF with Capillary LC and Conventional UPLC

TSQ Vantage-Agilent 1290 UHPLC (LC-MS)

Thermo Orbitrap Fusion with Easy Nano LC II

Sciex QTRAP 6500 MS/MS with Shimadzu UPLC

Sciex QTRAP 5500 MS/MS with Shimadzu UPLC

Thermo LTQ XL with Waters UPLC

Thermo Fleet

GCMS single Quad

GCMS Triple Quad

 

Instruments for Proteomics / Protein Characterization / Targeted Metabolomics / Untargeted Metabolomcis / HRMS

Thermo Q-Exactive

 

SCIEX QTRAP 6500 MS/MS

The combination of LC separation, high resolution MS and MS2 analysis should provide unambiguous and precise characterization of different classes of lipids in biological samples. Q-Exactive MS with its mass resolution of 140,000 at 400 m/z and mass accuracy of 1-2 ppm is optimized for lipidomics and metabolomics experiments.  HCD fragmentation using stepped normalised collision energies is an excellent tool for identification and quantitation of different classes of lipids.

 

The Sciex QTRAP 6500 builds on the capabilities of the 5500 series with improved sensitivity and linear dynamic range. One of the best is class triple-quadrupole MS instrument for the targeted quantitative analysis with MS3 fragemntation that enable for identification. It features the advanced IonDrive technology, which increases ion production & transmission efficiency. It also equipped with the differnetial ion-mobility technology.
 

 

  

Thermo Orbitrap Fusion with Easy Nano LC II

 

Sciex QTRAP 5500 MS/MS

The Orbitrap Fusion is a state-of-the-art hybrid mass spectrometer that integrates quadrupole, linear ion trap, and Orbitrap mass analyzers. It offers unparalleled flexibility and performance, with ultra-high resolution and accurate mass measurements. This instrument excels in deep proteome analysis, post-translational modification studies, and high-throughput screening, providing comprehensive data for complex biological research.

 

The Sciex QTRAP 5500 is a triple quadrupole linear ion trap mass spectrometer known for its high sensitivity and fast scanning capabilities. It combines quantitative and qualitative analysis in a single run, making it a powerful tool for biomarker discovery, drug metabolism, and protein identification. Its advanced features enhance accuracy and reduce analysis time, improving overall workflow efficiency.
 

 

  

Thermo LTQ Orbitrap XL 

 

Sciex TripleTOF 5600

LTQ Orbitrap XL MS with its mass resolution of 140,000 at 400 m/z and mass accuracy of 1-2 ppm is optimized for lipidomics and metabolomics experiments. The combination of LC separation, high resolution MS and MS2 analysis provides unambiguous characterization of different classes of lipids in biological samples.  CID fragmentation using pulsed collision energies is an excellent tool for identification and quantitation of different classes of lipids.

 

The TripleTOF 5600+ System combines SCIEX's Triple Quad technology with an Accelerator TOF Analyzer, offering both nominal and accurate mass measurements on a single platform. It supports comprehensive qualitative exploration, rapid profiling, and high-resolution quantitation workflows. This system is ideal for pharmaceutical development, omics research, and analyzing unknown compounds. Its high-resolution, high-sensitivity data and fast acquisition rates make it a versatile and powerful tool for mass spectrometry.