InStem Mass spec facility for Qualitiative and Quantitative charactetization of Biomolecules 

Zeno TOF 7600 - Zeno TOF 7600 is coupled high flow UPLC for analysis of metabolite, Protein, Peptide and Lipids. The 7600 Zeno TOF having the power of Zeno trap in combination with the binary fragmention mechnism i.e. Collision induced dissociaton and  EAD ( Electron activated dissociation) is very useful for the characterization of biomolecules. The high resolution of the analyzer is well suited for the characterization of biosimilars, Biobetter and intact proteins.Due to zeno trapping of the ion the sensitivity of this platfrom is enhanced multiple fold and dynamic range for the quantitation has also got improved significantly.

Following Mass Spectrometry Platfroms are present in the BLiSc cluster institutes.

Thermo Q-Exactive

5600 Triple TOF with Capillary LC and Conventional UPLC

TSQ Vantage-Agilent 1290 UHPLC (LC-MS)

Thermo Orbitrap Fusion with Nano LC

Sciex QTRAP 6500 MS/MS with Water UPLC

Sciex QTRAP 5500 MS/MS with UPLC

Thermo LTQ XL with LC

Thermo Fleet

GCMS single Quad

GCMS Triple Quad

 

Instruments for Proteomics / Protein Characterization

Thermo Q-Exactive

 

SCIEX QTRAP 6500 MS/MS

The combination of LC separation, high resolution MS and MS2 analysis should provide unambiguous and precise characterization of different classes of lipids in biological samples. Q-Exactive MS with its mass resolution of 140,000 at 400 m/z and mass accuracy of 1-2 ppm is optimized for lipidomics and metabolomics experiments.  HCD fragmentation using stepped normalised collision energies is an excellent tool for identification and quantitation of different classes of lipids.

 

One of the best is class triple-quadrupole MS instrument for the targeted quantitative analysis with MS3 fragmentation that enable for identification as well. It also equipped with the differnetial ion mobility technology.
 

 

  

Agilent Nano HPLC

 

Advion Triversa NanoMate Robot 

This LC system with flow rate ranges from 100 nl/min to 1µl/min is suitable for high sensitivity MS detection and 2-dimensional separation.

 

Uses a chip-based technology for stable sample infusion at nano litre flow rate.
 

 

  

TSQ Vantage-Agilent 1290 UHPLC

 

Thermo LTQ Orbitrap XL 

The Thermo Scientific TSQ Vantage uses precision hyperbolic quadrupoles, which provide the best transmission and peak shape, the detection technology and ion source results. It is the most sensitive triple quadrupole instrument. Agilent’s 1290 infinity series UHPLC system interfaces to the mass spectrometer for the automated LC-MS/MRM analysis.

 

This MS with its mass resolution of 100,000 at 400 m/z and mass accuracy of 1-2 ppm is optimized for high throughput proteomics experiments. It is equipped with two fragmentation techniques, CID and PQD, making it an excellent tool for Metabolomics and Intact Mass Analysis.