Generation of OCIAD2 homozygous knockout (BJNhem20-OCIAD2-CRISPR-33) and heterozygous knockout (BJNhem20-OCIAD2-CRISPR-40) human embryonic stem cell lines using CRISPR-Cas9 mediated targeting.
| Title | Generation of OCIAD2 homozygous knockout (BJNhem20-OCIAD2-CRISPR-33) and heterozygous knockout (BJNhem20-OCIAD2-CRISPR-40) human embryonic stem cell lines using CRISPR-Cas9 mediated targeting. |
| Publication Type | Journal Article |
| Year of Publication | 2023 |
| Authors | Kamat K, Inamdar MS |
| Journal | Stem Cell Res |
| Volume | 67 |
| Pagination | 103026 |
| Date Published | 2023 Mar |
| ISSN | 1876-7753 |
| Keywords | CRISPR-Cas Systems, Embryonic Stem Cells, Gene Knockout Techniques, Human Embryonic Stem Cells, Humans, Karyotype, Neoplasm Proteins |
| Abstract | Ovarian Carcinoma Immunoreactive Antigen domain containing 2 (OCIAD2) was knocked out by targeting its exon 4 through CRISPR-Cas9 paired nickase strategy to generate two OCIAD2 knockout human embryonic stem cell lines- one homozygous (BJNhem20-OCIAD2-CRISPR-33) and one heterozygous (BJNhem20-OCIAD2-CRISPR-40) for mutant ociad2. Both lines maintain pluripotency, normal karyotype, and trilineage differentiation potential. |
| DOI | 10.1016/j.scr.2023.103026 |
| Alternate Journal | Stem Cell Res |
| PubMed ID | 36642056 |