TitleGeneration of OCIAD2 homozygous knockout (BJNhem20-OCIAD2-CRISPR-33) and heterozygous knockout (BJNhem20-OCIAD2-CRISPR-40) human embryonic stem cell lines using CRISPR-Cas9 mediated targeting.
Publication TypeJournal Article
Year of Publication2023
AuthorsKamat K, Inamdar MS
JournalStem Cell Res
Date Published2023 Mar
KeywordsCRISPR-Cas Systems, Embryonic Stem Cells, Gene Knockout Techniques, Human Embryonic Stem Cells, Humans, Karyotype, Neoplasm Proteins

Ovarian Carcinoma Immunoreactive Antigen domain containing 2 (OCIAD2) was knocked out by targeting its exon 4 through CRISPR-Cas9 paired nickase strategy to generate two OCIAD2 knockout human embryonic stem cell lines- one homozygous (BJNhem20-OCIAD2-CRISPR-33) and one heterozygous (BJNhem20-OCIAD2-CRISPR-40) for mutant ociad2. Both lines maintain pluripotency, normal karyotype, and trilineage differentiation potential.

Alternate JournalStem Cell Res
PubMed ID36642056