Assessment of the inherent chondrogenic potential of human articular cartilage-derived chondroprogenitors in pellet culture using a novel whole pellet processing approach.
| Title | Assessment of the inherent chondrogenic potential of human articular cartilage-derived chondroprogenitors in pellet culture using a novel whole pellet processing approach. |
| Publication Type | Journal Article |
| Year of Publication | 2022 |
| Authors | Johnson NNaveen, Amirtham SManickam, B Rani S, Sathishkumar S, Rebekah G, Vinod E |
| Journal | J Orthop |
| Volume | 31 |
| Pagination | 45-51 |
| Date Published | 2022 May-Jun |
| ISSN | 0972-978X |
| Abstract | Purpose: Cartilage-derived chondroprogenitors have been reported to possess the biological potential for cartilage repair. However, its inherent chondrogenic potential in pellet culture needs evaluation. In-vitro cartilage regeneration models based on pellet cultures have been employed to evaluate the chondrogenic potential of stem cells. Evaluation of the degree of differentiation routinely involves paraffin embedding, sectioning, and immunohistochemical staining of the pellet. However, since chondrogenic differentiation is commonly non-uniform, processing random sections could lead to inaccurate conclusions. The study aimed at assessing the inherent lineage bias of chondroprogenitors with and without chondrogenic induction, using a novel whole pellet processing technique. Methods: Human chondroprogenitors (n=3) were evaluated for MSC markers and processed in pellet cultures either with stromal medium (uninduced) or chondrogenic differentiation medium (induced) for 28 days. The whole pellets and the conventional paraffin-embedded sectioned pellets were subjected to Collagen type II immunostaining and assessed using confocal laser microscopy. The staining intensities of the whole pellet were compared to the paraffin sections and revalidated using qRT-PCR for COL2A1 expression. Results: Uninduced and induced pellets displayed Collagen type II in all the layers with comparable fluorescence intensities. COL2A1 expression in both pellets was comparable to confocal results. The study demonstrated that uninduced chondroprogenitors in pellet culture possess promising inherent chondrogenic potential. Confocal imaging of whole pellets displayed different degrees of chondrogenic differentiation in the entire pellet, thus its probable in-vivo behavior. Conclusion: The novel approach presented in this study could serve as an efficient in-vitro alternative for understanding translational application for cartilage repair. |
| DOI | 10.1016/j.jor.2022.03.007 |
| Alternate Journal | J Orthop |
| PubMed ID | 35368732 |
| PubMed Central ID | PMC8967706 |
Comments
https://linktr.ee/toonkor 이 정보 메시지를 공유 할 수있는 훌륭한 기능입니다. 이 블로그에 대한 지식에 깊은 인상을 받았습니다. 여러면에서 도움이됩니다. 다시 게시 해 주셔서 감사합니다.
Read here. https://mexicanpharmonline.com/# mexican mail order pharmacies
Consistency, quality, and care on an international level. https://mexicanpharmonline.shop/# medication from mexico pharmacy
Always delivering international quality. http://mexicanpharmonline.com/# mexican drugstore online
Get here. https://mexicanpharmonline.com/# reputable mexican pharmacies online
They consistently go above and beyond for their customers. http://mexicanpharmonline.com/# medication from mexico pharmacy
Always on the pulse of international healthcare developments. https://mexicanpharmonline.shop/# п»їbest mexican online pharmacies
Their worldwide pharmacists' consultations are invaluable. http://mexicanpharmonline.shop/# medication from mexico pharmacy
A pharmacy that truly understands international needs. https://mexicanpharmonline.shop/# mexican rx online
A true asset to our neighborhood. https://mexicanpharmonline.com/# purple pharmacy mexico price list
Are you searching for Independent Call Girls at Goa then we are here for you. We offer hot and delightful young call girls in Goa. https://www.agoaescort.com/ We, accordingly, declared that best female escorts in Goa photos Gallery are the best start to look for escort benefits in the public capital most profile range.
Our sensual and awesome collection of Goa call girls is simply out of this world. Not only in terms of beauty but our Goa call girls in Goa are well equipped with the skills and techniques to woo, seduce, pamper and flatter clients to heights of satisfaction. Our high class Goa call girls are apt for VIP clients and https://www.juliegoaescorts.net.in/ other modish clients that need quality escorts and experience.
ivermectin 1 cream: <a href=" http://stromectol24.pro/# ">buy ivermectin canada</a> - ivermectin uk coronavirus
Our Indirapuram Call Girls Service gives up a created accomplice and gathering little youngsters for redirection purposes. https://www.indirapuramescorts.com/ We have practical inclusion in a private intensity of the customers we gave energetic young hot call girls and sexy call girls directly to your Hotel Room
canadian pharmacies online: <a href=" http://canadapharmacy24.pro/# ">canadian pharmacy pro</a> - canadian pharmacy online
indian pharmacy paypal: <a href=" http://indiapharmacy24.pro/# ">buy prescription drugs from india</a> - best india pharmacy
reputable canadian pharmacy: <a href=" https://canadapharmacy24.pro/# ">canada pharmacy</a> - canadian pharmacies compare
canadian pharmacy: <a href=" http://canadapharmacy24.pro/# ">canadian pharmacy pro</a> - pharmacies in canada that ship to the us
top 10 online pharmacy in india: <a href=" http://indiapharmacy24.pro/# ">top online pharmacy india</a> - online pharmacy india
minocycline: <a href=" http://stromectol24.pro/# ">stromectol ivermectin buy</a> - buy stromectol
my canadian pharmacy reviews: <a href=" https://canadapharmacy24.pro/# ">safe canadian pharmacy</a> - canada drugs online review
ivermectin buy online: <a href=" http://stromectol24.pro/# ">stromectol tablets buy online</a> - buy minocycline 50mg
indianpharmacy com: <a href=" http://indiapharmacy24.pro/# ">buy prescription drugs from india</a> - buy prescription drugs from india
https://digitaldicemedia.com/ What a fantabulous post this has been. Never seen this kind of useful post. I am grateful to you and expect more number of posts like these. Thank you very much
india pharmacy mail order: <a href=" http://indiapharmacy24.pro/# ">mail order pharmacy india</a> - india pharmacy mail order
www canadianonlinepharmacy: <a href=" https://canadapharmacy24.pro/# ">best pharmacy online</a> - canadian world pharmacy
minocycline 100mg for sale: <a href=" http://stromectol24.pro/# ">buy ivermectin canada</a> - stromectol pills
canadian pharmacy no scripts: <a href=" http://canadapharmacy24.pro/# ">best pharmacy online</a> - canadianpharmacyworld com
best india pharmacy: <a href=" https://indiapharmacy24.pro/# ">indianpharmacy com</a> - indian pharmacy online
canadian pharmacy online store: <a href=" http://canadapharmacy24.pro/# ">Pharmacies in Canada that ship to the US</a> - canadadrugpharmacy com
reputable indian pharmacies: <a href=" http://indiapharmacy24.pro/# ">pharmacy website india</a> - reputable indian online pharmacy
online pharmacy india: <a href=" http://indiapharmacy24.pro/# ">Online medicine order</a> - п»їlegitimate online pharmacies india
п»їwhere to buy stromectol online: <a href=" https://stromectol24.pro/# ">stromectol tablets buy online</a> - minocycline 50mg tablets for human
dynacin minocycline: <a href=" https://stromectol24.pro/# ">stromectol ivermectin buy</a> - ivermectin 6 tablet
canadian pharmacy meds: <a href=" https://canadapharmacy24.pro/# ">reputable canadian online pharmacies</a> - canadian pharmacy 24 com
stromectol price usa: <a href=" https://stromectol24.pro/# ">stromectol order online</a> - buy minocycline 50 mg for humans
canadian drugs online: <a href=" http://canadapharmacy24.pro/# ">canada pharmacy</a> - canadian online drugs
paxlovid buy: <a href=" https://paxlovid.bid/# ">paxlovid best price</a> - paxlovid cost without insurance
how much is valtrex generic: <a href=" https://valtrex.auction/# ">valtrex antiviral drug</a> - buy generic valtrex online
minocycline mr: <a href=" https://stromectol.icu/# ">ivermectin 3mg</a> - ivermectin oral
buy clopidogrel bisulfate: <a href=" http://plavix.guru/# ">buy plavix</a> - plavix medication
Paxlovid buy online: <a href=" https://paxlovid.bid/# ">paxlovid best price</a> - paxlovid pill
Do you want to change your life with our vlone now, our <a href="https://vlone.today/">Vlone</a> sale for you with 50% discount online.
A new establishment has recently been inaugurated by Papa Louie: an authentic Italian pizzeria. As the individual in command, it is your responsibility to receive orders, prepare the pizza base, and construct the garnishes for every consumer. Ensure that the pizza garnishes are added in the correct sequence. Ultimately, perhaps you will obtain a slice for your own consumption... https://papaspizzeriagame.github.io/
This content is written very well. Your use of formatting when making your points makes your observations very clear and easy to understand. Thank you. https://suika.gg/
Thanks for sharing this information. I have shared this link with others keep posting such information.
See more details about Tree Pruning Charlottesville at https://www.metcalftree.com/treepruning.html
Morse Code Translator - Morse Decoder
Morse code translator is a translator that lets anyone translate text to Morse code and decode Morse code to text easily. With the online Morse code translator, ...
https://www.morsecode-translator.com/
https://www.morsecode-translator.com/
https://www.morsecode-translator.com/
https://www.morsecode-translator.com/
https://www.morsecode-translator.com/
https://www.morsecode-translator.com/
Speedy service with a smile! https://mexicanpharmonline.shop/# buying prescription drugs in mexico online
Nyt Wordle is a popular word game that has taken the globe by storm. It’s a fun and addictive way to test your vocabulary skills and challenge yourself with word puzzles. But what exactly is New York Times Wordle Today? In simple terms, Nyt Wordle is an online game where players try to guess a five-letter target word within six tries. Each time you make a guess, the game provides feedback on which letters are correct and in the right position or if any letters are correct but in the wrong position. The beauty of Nyt Wordle lies in its simplicity. You don’t need complicated rules or instructions to get started. Just open up the website or app, and you’re ready to begin guessing words! The interface is clean and user-friendly, making it accessible for players of all ages.
<a href="https://nytwordle.today/">Nyt Wordle</a>
https://nytwordle.today/