As scientists, we would like to understand how a molecule works at atomic level and obtaining a structure is ultimate goal in this process. Electron microscopy has the power to obtain atomic resolution structure of macromolecules and in recent times, the advances in the hardware of electron microscopes, detectors and softwares has made the technique very powerful and many macromolecular structures, which previously were difficult to study structurally can now be studied. In electron cryomicroscopy, macromolecules in solution are frozen rapidly and then imaged with electrons.

The images, which are projections of the molecule of interest are then averaged and reconstructed to obtain a high-resolution map . As the molecules are frozen from a solution the possibility to get multiple conformational or structural states is an added advantge with cryoEM.