Liposomes have emerged as an indispensable tool in biomedical applications due to their versatility, biocompatibility, and capacity to enhance various agents’ delivery and therapeutic efficacy. Their expanding role in drug and gene delivery, vaccine development, and diagnostics underscores their significance in advancing modern medical treatments and personalized healthcare. The ability to engineer liposomes to meet specific therapeutic requirements highlights their critical importance in contemporary medicine, providing innovative solutions for treating and diagnosing diverse diseases. The ongoing advancement of liposomal preparation methods is essential for addressing the limitations of traditional approaches and realizing the full therapeutic potential of liposomes. These advancements are poised to yield more efficient, scalable, and customizable liposomal formulations, facilitating the development of next-generation therapies that are safer, more effective, and more accessible. This article provides a detailed analysis of the progress in lipid-based nanotechnology preparation methods, and it examines the fundamental properties and classification of liposomes, their historical context, and the evolution of liposome research. Additionally, it explores various liposome preparation methods, including conventional techniques such as thin-film hydration and emerging novel approaches. Overall, this manuscript offers a comprehensive overview of the pivotal role that advancements in lipid-based nanotechnology preparation methods play in therapeutic innovation.