Department of Biotechnology
inStem (Institute for Stem Cell Science and Regenerative Medicine)

Characterization of FOXO3-14-3-3 Interaction by Isothermal Titration Calorimetry.

Publication Type

Journal Article

Date of Publication

January 1, 2025

Journal

Methods in molecular biology (Clifton, N.J.)

Volume/Issue

2871

ISSN

1940-6029

Isothermal titration calorimetry (ITC) is a well-established and convenient label-free technique for measuring biomolecular interactions in aqueous solutions. ITC enables accurate measurement of the affinities and thermodynamic parameters, such as changes in Gibbs energy, enthalpy, and entropy, which help dissect the binding mechanisms. ITC is commonly used to study protein-protein, protein-peptide, protein-DNA, and small-molecule inhibitor binding to target proteins. ITC can determine binding affinities ranging from nM to low-mM. This chapter provides a detailed protocol to investigate the binding of FOXO3 peptide to 14-3-3ε using ITC.

Alternate Journal

Methods Mol Biol

PubMed ID

39565582

Authors

Thazhe Kootteri Prasad
Sneha Bairy
Neelagandan Kamariah

Keywords

Humans
14-3-3 Proteins
Protein Binding
Forkhead Box Protein O3
Calorimetry
Thermodynamics