Generation of OCIAD2 homozygous knockout (BJNhem20-OCIAD2-CRISPR-33) and heterozygous knockout (BJNhem20-OCIAD2-CRISPR-40) human embryonic stem cell lines using CRISPR-Cas9 mediated targeting.

Publication Type

Journal Article

Date of Publication

March 1, 2023

Journal

Stem cell research

Volume/Issue

ISSN

1876-7753

Ovarian Carcinoma Immunoreactive Antigen domain containing 2 (OCIAD2) was knocked out by targeting its exon 4 through CRISPR-Cas9 paired nickase strategy to generate two OCIAD2 knockout human embryonic stem cell lines- one homozygous (BJNhem20-OCIAD2-CRISPR-33) and one heterozygous (BJNhem20-OCIAD2-CRISPR-40) for mutant ociad2. Both lines maintain pluripotency, normal karyotype, and trilineage differentiation potential.

DOI

10.1016/j.scr.2023.103026

Alternate Journal

Stem Cell Res

PubMed ID

36642056

Authors

Kajal Kamat

Maneesha S Inamdar

Keywords

Humans

CRISPR-Cas Systems

Human Embryonic Stem Cells

Neoplasm Proteins

Gene Knockout Techniques

Karyotype

Embryonic Stem Cells