Campus-wide Stem Cell Facility (SCF) is the core facility to provide a platform for human pluripotent stem cell (hPSCs) research on campus.

Objectives of the SCF

To provide appropriate training for users to culture human embryonic stem cells (hESCs) and human induced pluripotent cells (hiPSCs).

To facilitate users to grow and conduct experiments with Human Pluripotent Stem Cells (hPSCs).

Stem Cell Core

Our mission is to provide state of the art services to conduct cutting edge human pluripotent stem cell research. Please submit your request via link: https://www.instem.res.in/stemcellfacility/user/login

More details on the below mentioned services could be found here. (pdf(link is external)) Please contact core for the cost of services provided.

• Fibroblast isolation & culture
• Inactivated fibroblast preparation
• PBMC isolation
• PBMC expansion & cryopreservation
• Reprogramming (human: from fibroblasts, PBMCs)
• hiPSC expansion & banking
• Characterization of established hiPSC lines
• Consultations for grant writing, protocol review, experiment design and analysis
• Monthly Facility usage
• Others: Mycoplasma detection

Introducong our new service of “Biorepository” where stakeholders from both academia and industry could now deposit and request human stem cell lines. Write to us for more details.

Upcoming services:

• Workshops and hands-on-trainings
• Genome editing
• Differentiation (monolayer & organoids)
• Drug screening/ Toxicity assessment/ Pharmacogenomics
• mESC/iPSC reprogramming, culture & differentiation

Acknowledgements

The advisory committee recommends the users of Stem cell Facility to acknowledge the facility in their scientific publications, posters and meetings. The facility can be acknowledged as “Stem cell Facility at Bangalore Life Science Cluster”. Please send a copy of your publication in which stem cell facility is acknowledged to stemcellcore@instem.res.in(link sends e-mail).

For submitting manuscripts and grants that have made use of services or resources from this core, PLEASE ACKNOWLEDGE SUPPORT by inclusion of the following information:

• “This work was supported by the Stem Cell Core at Bangalore Life Science Cluster.“
• Please cite the Flagship program (DBT, GoI) (BT/PR17316/MED/31/326/2015) in all publications and grants related to services from stem cell core usage.

ETHICAL Clearance to be mentioned in your Materials and Methods of all your publications involving the use of stem cells: Recommended statement would be: “All experiments were performed according to the approved Instem or NCBS IHEC, IBSC and IC-SCR guidelines (all those applicable)”.

Collaborative Research

Stem cell core welcomes ideas for collaborative custom projects. Appropriate to the scientific and technical contribution, Core personnel would be co-authors in the resulting manuscripts.