%0 Journal Article %J J Phys Chem B %D 2022 %T Decoding the Kinetic Pathways toward a Lipid/DNA Complex of Alkyl Alcohol Cationic Lipids Formed in a Microfluidic Channel. %A Mukherjee, Dipanjan %A Hasan, Md Nur %A Ghosh, Ria %A Ghosh, Gourab %A Bera, Arpan %A Prasad, Sujanthi Easwara %A Hiwale, Ankita %A Vemula, Praveen K %A Das, Ranjan %A Pal, Samir Kumar %X

Complexes of cationic liposomes with DNA have emerged as promising nonviral vectors for delivering genetic information into cells for gene therapy. Kinetics of the liposome/DNA complex (lipoplex) formation on a millisecond time scale are studied by monitoring time evolution of fluorescence of 8-anilino-1-naphthalene sulfonic acid (ANS) and ethidium bromide (EtBr) in a continuous flow microfluidic channel coupled to a fluorescence microscope. The formation of lipoplexes between calf thymus DNA and liposomes based on two novel cationic lipids (Lip1810 and Lip1814) are found to follow a two-step process with kinetic constants for the Lip1814/DNA complex ( = 1120-1383 s, = 0.227-1.45 s) being significantly different from those ( = 68.53-98.5 s, = 32.3-60.19 s) corresponding to formation of the Lip1810/DNA complex. The kinetic pathway leading to the formation of Lip1814/DNA complex is whereas the formation of Lip1810/DNA complex occurs by a . The observed difference in the kinetics of lipoplex formation likely originates from different structures of the lipid/DNA complexes.

%B J Phys Chem B %V 126 %P 588-600 %8 2022 Jan 27 %G eng %N 3 %R 10.1021/acs.jpcb.1c07263