TY - JOUR T1 - Chemically diverse small molecule fluorescent chemosensors for copper ion JF - Coordination Chemistry Reviews Y1 - 2018 A1 - Gandhi Sivaraman A1 - Murugan Iniya A1 - Thangaraj Anand A1 - Niranjan G. Kotla A1 - Omprakash Sunnapu A1 - Subramanian Singaravadivel A1 - Akash Gulyani A1 - Duraisamy Chellappa KW - Chemosensors KW - Fluorescence KW - Live cell imaging KW - Small molecules KW - Turn-off KW - Turn-on VL - 357 UR - http://www.sciencedirect.com/science/article/pii/S0010854517306537 ER - TY - JOUR T1 - Facile Synthesis of Highly Sensitive, Red‐Emitting, Fluorogenic Dye for Microviscosity and Mitochondrial Imaging in Embryonic Stem Cells JF - ChemistrySelect Y1 - 2017 A1 - Raja Sufi O. A1 - Sivaraman Gandhi A1 - Mukherjee Ananya A1 - Duraisamy Chellappa A1 - Gulyani Akash KW - Embryonic Stem Cells KW - Live cell imaging KW - Microviscosity KW - Mitochondrial fluorescent probe KW - Photoisomerization AB -

Abstract Bright, sensitive fluorescent probes that respond to changes in the cellular microenvironment are extremely valuable for imaging cellular dynamics. We report a simple, one‐step synthesis of a new hemicaynine (HC‐1) dye as a sensitive, red‐emitting (λmax‐610 nm) fluorogenic probe for micro‐viscosity and local order in diverse environments, including live cells. HC‐1 responds to increasing micro‐viscosity through changes in fluorescence intensity and lifetime, and is sensitive enough to report dynamic micellar self‐assembly. While HC‐1 shows properties of a molecular ‘rotor’, time‐dependent density functional theoretical analysis reveals that in HC‐1, an inhibition of photo‐isomerization in viscous environment is the likely cause of fluorescence enhancement. HC‐1 localizes to mitochondria in live cells and responds to mitochondrial ordering through a significant increase in fluorescence. Strikingly, we show that HC‐1 is also a sensitive probe for the spatial heterogeneity of mitochondrial organization in embryonic stem cells as well as dynamic remodeling of the mitochondria in early‐differentiated cells.

VL - 2 UR - https://onlinelibrary.wiley.com/doi/abs/10.1002/slct.201700463 ER -