@article {1202,
	title = {Facile Synthesis of Highly Sensitive, Red-Emitting, Fluorogenic Dye for Microviscosity and Mitochondrial Imaging in Embryonic Stem Cells},
	journal = {ChemistrySelect},
	volume = {2},
	year = {2017},
	pages = {4609-4616},
	abstract = {<p>Abstract Bright, sensitive fluorescent probes that respond to changes in the cellular microenvironment are extremely valuable for imaging cellular dynamics. We report a simple, one-step synthesis of a new hemicaynine (HC-1) dye as a sensitive, red-emitting (λmax-610 nm) fluorogenic probe for micro-viscosity and local order in diverse environments, including live cells. HC-1 responds to increasing micro-viscosity through changes in fluorescence intensity and lifetime, and is sensitive enough to report dynamic micellar self-assembly. While HC-1 shows properties of a molecular {\textquoteleft}rotor{\textquoteright}, time-dependent density functional theoretical analysis reveals that in HC-1, an inhibition of photo-isomerization in viscous environment is the likely cause of fluorescence enhancement. HC-1 localizes to mitochondria in live cells and responds to mitochondrial ordering through a significant increase in fluorescence. Strikingly, we show that HC-1 is also a sensitive probe for the spatial heterogeneity of mitochondrial organization in embryonic stem cells as well as dynamic remodeling of the mitochondria in early-differentiated cells.</p>
},
	keywords = {Embryonic Stem Cells, Live cell imaging, Microviscosity, Mitochondrial fluorescent probe, Photoisomerization},
	doi = {10.1002/slct.201700463},
	url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/slct.201700463},
	author = {Raja Sufi O. and Sivaraman Gandhi and Mukherjee Ananya and Duraisamy Chellappa and Gulyani Akash}
}
