@article {2425,
	title = {Decoding the Kinetic Pathways toward a Lipid/DNA Complex of Alkyl Alcohol Cationic Lipids Formed in a Microfluidic Channel.},
	journal = {J Phys Chem B},
	volume = {126},
	year = {2022},
	month = {2022 Jan 27},
	pages = {588-600},
	abstract = {<p>Complexes of cationic liposomes with DNA have emerged as promising nonviral vectors for delivering genetic information into cells for gene therapy. Kinetics of the liposome/DNA complex (lipoplex) formation on a millisecond time scale are studied by monitoring time evolution of fluorescence of 8-anilino-1-naphthalene sulfonic acid (ANS) and ethidium bromide (EtBr) in a continuous flow microfluidic channel coupled to a fluorescence microscope. The formation of lipoplexes between calf thymus DNA and liposomes based on two novel cationic lipids (Lip1810 and Lip1814) are found to follow a two-step process with kinetic constants for the Lip1814/DNA complex ( = 1120-1383 s, = 0.227-1.45 s) being significantly different from those ( = 68.53-98.5 s, = 32.3-60.19 s) corresponding to formation of the Lip1810/DNA complex. The kinetic pathway leading to the formation of Lip1814/DNA complex is whereas the formation of Lip1810/DNA complex occurs by a . The observed difference in the kinetics of lipoplex formation likely originates from different structures of the lipid/DNA complexes.</p>
},
	issn = {1520-5207},
	doi = {10.1021/acs.jpcb.1c07263},
	author = {Mukherjee, Dipanjan and Hasan, Md Nur and Ghosh, Ria and Ghosh, Gourab and Bera, Arpan and Prasad, Sujanthi Easwara and Hiwale, Ankita and Vemula, Praveen K and Das, Ranjan and Pal, Samir Kumar}
}
