@article {2325,
	title = {Isolation and Quantification of Mouse γδT-cells  and .},
	journal = {Bio Protoc},
	volume = {11},
	year = {2021},
	month = {2021 Sep 05},
	pages = {e4148},
	abstract = {<p>The skin plays an important role in protecting the body from pathogens and chemicals in the external environment. Upon injury, a healing program is rapidly initiated and involves extensive intercellular communication to restore tissue homeostasis. The deregulation of this crosstalk can lead to abnormal healing processes and is the foundation of many skin diseases. A relatively overlooked cell type that nevertheless plays critical roles in skin homeostasis, wound repair, and disease is the dendritic epidermal T cells (DETCs), which are also called γδT-cells. Given their varied roles in both physiological and pathological scenarios, interest in the regulation and function of DETCs has substantially increased. Moreover, their ability to regulate other immune cells has garnered substantial attention for their potential role as immunomodulators and in immunotherapies. In this article, we describe a protocol to isolate and culture DETCs and analyse them within the skin. These approaches will facilitate the investigation of their crosstalk with other cutaneous cells and the mechanisms by which they influence the status of the skin. Graphic abstract: Overall workflow to analyse DETCs and .</p>
},
	issn = {2331-8325},
	doi = {10.21769/BioProtoc.4148},
	author = {Rana, Isha and Badarinath, Krithika and Zirmire, Ravindra K and Jamora, Colin}
}
@article {1156,
	title = {Stimulation of hair follicle stem cell proliferation through an IL-1 dependent activation of γδT-cells.},
	journal = {Elife},
	volume = {6},
	year = {2017},
	month = {2017 Dec 04},
	abstract = {<p>The cutaneous wound-healing program is a product of a complex interplay among diverse cell types within the skin. One fundamental process that is mediated by these reciprocal interactions is the mobilization of local stem cell pools to promote tissue regeneration and repair. Using the ablation of epidermal caspase-8 as a model of wound healing in , we analyzed the signaling components responsible for epithelial stem cell proliferation. We found that IL-1α and IL-7 secreted from keratinocytes work in tandem to expand the activated population of resident epidermal γδT-cells. A downstream effect of activated γδT-cells is the preferential proliferation of hair follicle stem cells. By contrast, IL-1α-dependent stimulation of dermal fibroblasts optimally stimulates epidermal stem cell proliferation. These findings provide new mechanistic insights into the regulation and function of epidermal cell-immune cell interactions and into how components that are classically associated with inflammation can differentially influence distinct stem cell niches within a tissue.</p>
},
	issn = {2050-084X},
	doi = {10.7554/eLife.28875},
	author = {Lee, Pedro and Gund, Rupali and Dutta, Abhik and Pincha, Neha and Rana, Isha and Ghosh, Subhasri and Witherden, Deborah and Kandyba, Eve and MacLeod, Amanda and Kobielak, Krzysztof and Havran, Wendy L and Jamora, Colin}
}
