@article {3343,
	title = {Initiation of wound healing is regulated by the convergence of mechanical and epigenetic cues.},
	journal = {PLoS Biol},
	volume = {20},
	year = {2022},
	month = {2022 09},
	pages = {e3001777},
	abstract = {<p>Wound healing in the skin is a complex physiological process that is a product of a cell state transition from homeostasis to repair. Mechanical cues are increasingly being recognized as important regulators of cellular reprogramming, but the mechanism by which it is translated to changes in gene expression and ultimately cellular behavior remains largely a mystery. To probe the molecular underpinnings of this phenomenon further, we used the down-regulation of caspase-8 as a biomarker of a cell entering the wound healing program. We found that the wound-induced release of tension within the epidermis leads to the alteration of gene expression via the nuclear translocation of the DNA methyltransferase 3A (DNMT3a). This enzyme then methylates promoters of genes that are known to be down-regulated in response to wound stimuli as well as potentially novel players in the repair program. Overall, these findings illuminate the convergence of mechanical and epigenetic signaling modules that are important regulators of the transcriptome landscape required to initiate the tissue repair process in the differentiated layers of the epidermis.</p>
},
	keywords = {Biomarkers, Caspase 8, Cues, Epigenesis, Genetic, Wound Healing},
	issn = {1545-7885},
	doi = {10.1371/journal.pbio.3001777},
	author = {Bhatt, Tanay and Dey, Rakesh and Hegde, Akshay and Ketkar, Alhad Ashok and Pulianmackal, Ajai J and Deb, Ashim P and Rampalli, Shravanti and Jamora, Colin}
}
@article {1986,
	title = {Sustained Secretion of the Antimicrobial Peptide S100A7 Is Dependent on the Downregulation of Caspase-8.},
	journal = {Cell Rep},
	volume = {29},
	year = {2019},
	month = {2019 Nov 26},
	pages = {2546-2555.e4},
	abstract = {<p>Antimicrobial peptides (AMPs) are the body{\textquoteright}s natural innate immune defense against a spectrum of pathogens and can also modulate cell proliferation, chemotaxis, angiogenesis, wound healing, and immune cell activity. Harnessing these diverse functions for prophylactic use is contingent upon understanding the regulatory mechanisms governing their unconventional secretion from cells. Analysis of the secretion of S100A7 (Psoriasin), an abundant AMP stored in differentiated keratinocytes of the skin, has revealed an unexpected biphasic secretory response to bacterial exposure. The core components regulating S100A7 secretion are NFκB/p38MAPK, caspase-1, and interleukin (IL)-1α. The initial activation of this core machinery is mediated by Toll-like receptor signaling, whereas the chronic response is mediated by Caspase-8 downregulation. Interestingly, there is a concomitant downregulation of Caspase-8 in inflammatory skin diseases wherein S100A7 is constitutively released. These results highlight the potential of targeting these components to control the release of AMPs from the skin in both homeostatic and disease conditions.</p>
},
	issn = {2211-1247},
	doi = {10.1016/j.celrep.2019.10.090},
	author = {Bhatt, Tanay and Bhosale, Aishwarya and Bajantri, Bhavya and Mathapathi, Mruthyunjaya Swamy and Rizvi, Abrar and Scita, Giorgio and Majumdar, Amitabha and Jamora, Colin}
}
